To determine whether downregulation of PRDX1 inhibits the growth of CRC by inducing ferroptosis through suppressing NRF2-mediated transcriptional regulation of antioxidant proteins in vivo, we knocked down PRDX1 in murine CRC cell line CT26 and established subcutaneous xenograft tumors using CT26PRDX1-KD cells in the presence or absence of the NRF2 activator TBHQ or ferroptosis inhibitor Fer-1. Here, PRDX1 is linked to colorectal carcinoma.