To further verify whether silencing of PRDX1 induced cancer cell death was also due to ferroptosis, we treated HCT116PRDX1-KD and SW620PRDX1-KD cells with either apoptosis inhibitor (z-VAD) or ferroptosis inhibitor (Fer-1) and then assessed cell viability (Fig. 2D). This evidence concerns the gene PRDX1 and cancer.