The comparison of the groups according to the NAFLD grade revealed no significant difference in age or presence of gallstone, but there were statistically significant differences in weight, BMI, ALT, AST, HDL, LDL, TG, total cholesterol, HbA1c and FBG, vitamin D, obesity class, DM, and HT (p <.05) (Table 3). The gene discussed is GPT; the disease is gallstones.