In the multivariate analysis, ALT, AST, LDL, total cholesterol, and FBG showed statistically significant associations with NAFLD. As a result of the ROC curve analysis performed to obtain the cut-off value, it was observed that the cut-off value for AST was 22 U/L, the cut-off value for ALT was 29 U/L, the cut-off value for Total cholesterol was 199 mg/dL, the cut-off value for FBG was 100 mg/dL, and the cut-off value for LDL was 178 mg/dL (see Table 4 and Figure 1). The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.