FASN and metabolic dysfunction-associated steatotic liver disease: Among these lipid synthesis related proteins, we noted that FASN sites including FASN_K1465, FASN_K1491, FASN_K1860, FASN_K1886, FASN_K826, FASN_K852, FASN_K2447, FASN_K2473, FASN_K78, FASN_K104, FASN_K1780, FASN_K1806, FASN_K86, FASN_K112, FASN_K107, FASN_K133, FASN_K1274, FASN_K1300, FASN_K1191, FASN_K1217, FASN_K96 and FASN_K122 were extensively ubiquitinated by High BCAA feeding, which is concomitant with our RNA-Seq, Western blot and ELISA data, demonstrating FASN ubiquitination degradation induced by High BCAA feeding is necessary for the amelioration of NAFLD.