AGT and inflammation: For example, HEK-293 cells deficient in the TRPM7α-kinase domain secrete the proinflammatory mediators ROS, ICAM-1, Cox-2, and PAI-1 in response to aldosterone stimulation, resulting in proinflammatory phenotypes [36]; TRPM7 kinase-deficient heterozygous mice with Ang II infusion present increased blood pressure, vascular dysfunction, cardiovascular inflammation and fibrosis [37]; and the expression of proinflammatory factors, such as Ang II and reactive oxygen species (ROS), increases TRPM7 expression in VSMCs [38].