To address if SIRT1 is intrinsically impacted in DCs during obesity, we first demonstrated that the Sirt1 gene expression was downregulated in bone marrow-derived dendritic cells (BMDCs) from mice under a high-fat diet (HFD) (hereafter, BMDCs/HFD; Extended data Fig. 1a, b; Fig. 1A). This evidence concerns the gene SIRT1 and obesity due to melanocortin 4 receptor deficiency.