From these datasets, the 48,638 cells were annotated into seven cell types, including immune cells, which include T cells (CD3D, CD3E), B cells (MS4A1, CD79A), NK cells (FGFBP2, KLRD1), and macrophages (LYZ, CD68, CD14), and non-immune cells, which include melanoma cells (MLANA, PMEL, MITF, DCT), endothelial cells (VWF, PECAM1), and fibroblast cells (COL1A1, COL3A1) (Fig. 5D, Supplementary Fig. 5B). The gene discussed is VWF; the disease is melanoma.