From these datasets, the 48,638 cells were annotated into seven cell types, including immune cells, which include T cells (CD3D, CD3E), B cells (MS4A1, CD79A), NK cells (FGFBP2, KLRD1), and macrophages (LYZ, CD68, CD14), and non-immune cells, which include melanoma cells (MLANA, PMEL, MITF, DCT), endothelial cells (VWF, PECAM1), and fibroblast cells (COL1A1, COL3A1) (Fig. 5D, Supplementary Fig. 5B). This evidence concerns the gene CD3D and melanoma.