We adjusted primers from a previously developed high-resolution melting (HRM) assay33 to capture IS5-catA1 and the truncated catB3∆443–633, and demonstrated that they can be clearly distinguished from the wild-type genes (Supplementary Fig. 5), highlighting the potential of this molecular diagnostic test for CHL resistance to discriminate between functional and non-functional cat genes. Here, CAT is linked to classic Hodgkin lymphoma.