To determine if intracellular Ca2+ efflux is sufficient to induce T3SS-dependent LTB4 synthesis, BMNs were pre-treated with EGTA to chelate extracellular Ca2+ prior to infection with Y. pestis T3E –if intracellular Ca2+ efflux is sufficient for cPLA2 and 5-LOX activation, then EGTA should not inhibit LTB4 synthesis. The gene discussed is PLA2G4A; the disease is infection.