The top 10 signaling pathways with the most significant P values were Relaxin signaling pathway, endocrine resistance pathway, diabetic cardiomyopathy pathway, estrogen signaling pathway, fluid shear stress and atherosclerotic pathway, cancer pathway, Chagas disease pathway, C‐type lectin receptor pathway, vascular endothelial growth factor signaling pathway and prolactin signaling pathway (Figure 4C). The gene discussed is VEGFA; the disease is Chagas disease.