To validate the RNA sequencing results, we performed qPCR and ELISA assays and found that CXCL12 expression was upregulated in human and mouse PRP19 knockdown HCC cells (Figure 3E,F) and down‐regulated in PRP19 overexpressed HCC cells (Figure S3D, Supporting Information). The gene discussed is PRPF19; the disease is hepatocellular carcinoma.