In order to detect the expression of N4BP3 in the human colon tissue, we collected 20 surgical pathological paraffin sections from CD patients and 20 normal control colon tissue from colon cancer patients who received surgery for tumor removal (sections taken at a distance of over 15 cm from the tumor edge), and then detected the expression of N4BP3 by IHC assay. The gene discussed is N4BP3; the disease is malignant colon neoplasm.