CD44 and neoplasm: To confirm these results, we analyzed changes in RNA stability after stopping transcription by adding actinomycin D. The results showed that the knockdown of ZCCHC24 decreased the stability of mRNAs derived from genes important for tumorigenicity, tumor progression, and treatment resistance, such as ZEB1, NRP1, CD44, NOTCH2, CDH11, ADAMTS1, and JAG1 (Fig. 2E; Appendix Fig. S5).