Motor neurons are particularly susceptible to calcium overload due to their inherent low levels of calcium buffering proteins like parvalbumin and calbindin compared to other neuron types41 We extended our analysis on the expression of P2X7R to include other motor neuron populations, that have been shown to be selectively resistant to cell death both pathologically and clinically in ALS, specifically: the oculomotor and Onuf’s nucleus motor neurons. Here, CALB1 is linked to amyotrophic lateral sclerosis.