In Treg cell group, CD127 on CD28+CD4+(P = 0.021, OR = 1.039, 95%CI = 1.005~1.073), Secreting Treg AC (P = 0.024, OR = 1.019, 95%CI = 1.002~1.036), CD25hi CD45RA-CD4 not Treg AC (P = 0.020, OR = 1.023, 95%CI = 1.003~1.043) and CD4 Treg AC (P = 0.013, OR = 1.047, 95%CI = 1.009~1.087) were positively correlated with breast cancer. Here, CD28 is linked to breast cancer.