TBX1 and neoplasm: The results show that 15 clusters are defined as 11 cell types, with cluster0 as epithelial cells, cluster1, and cluster9 as tissue stem cells, cluster 3,5 are epithelial cells, cluster 4 is T cell, cluster 8 is fibroblasts, and so on (Fig 2D).Since CAFs is the main component of TME and can promote tumor progression, we chose CAFs module, marker genes in cluster8 for function enrichment analysis, the results shown that these genes are enriched in multiple pathways and biological processes associated with CAFs (Fig 2E and 2F).