It has been reported that circulating levels of cholesterol, fatty acids, glucose, and insulin increased sequentially in mice after HFD feeding.[17] HFHS‐fed mice have a significant increase in blood cholesterol levels.[27] Ob/ob mice and db/db mice have higher blood cholesterol and fatty acid levels compared to control mice.[28] Therefore, the increased expression of hepatic Gk in these mouse models of NAFL may result from the elevation of circulating cholesterol and fatty acids. This evidence concerns the gene INS and non-alcoholic fatty liver.