Our study showed that knockdown of hepatic Gk in mice with HFD‐induced hepatic steatosis significantly increased serum levels of glycerol, reduced the expression of Srebp‐1c and its target lipogenic genes as well as TG synthesis genes in the liver, lowered serum lipid levels, and decreased hepatic TG deposition. This evidence concerns the gene SREBF1 and Hepatic steatosis.