GK and non-alcoholic fatty liver: It has been reported that circulating levels of cholesterol, fatty acids, glucose, and insulin increased sequentially in mice after HFD feeding.[17] HFHS‐fed mice have a significant increase in blood cholesterol levels.[27] Ob/ob mice and db/db mice have higher blood cholesterol and fatty acid levels compared to control mice.[28] Therefore, the increased expression of hepatic Gk in these mouse models of NAFL may result from the elevation of circulating cholesterol and fatty acids.