Then, to verify the role of AR nuclear translocation in AKR1C1 expression and BC progression, we treated the HCC1806 cells with 5α‐dihydrotestosterone (5α‐DHT), an AR nuclear translocation activator.[59] To demonstrate the effect of 5α‐DHT on AR nuclear translocation, we performed immunofluorescence and observed more colocalization between AR and nucleus in HCC1806 cells after treatment 5α‐DHT (0.1 μm and 1 μm) (Figure S6C, Supporting Information). This evidence concerns the gene AR and breast cancer.