We estimated HIV incidence for two scenarios, the first considering those two samples as missing information and the second considering them as long-standing infection, following our RITA, one due to a previous record in the Brazilian National CD4+/CD8+ T Lymphocyte Count and Viral Load Network Laboratory Test Control System (SISCEL) dated 12 months before the diagnosis in our study, and the second according to the T-CD4 cell count (Figure 1). This evidence concerns the gene CD4 and infection.