To obtain transcriptome level evidencethat may shed light on the HCC-selectivity and/or the enhanced potencyof the combination of STR-V-53 with anti-PD-1 antibody,we performed RNA sequencing (RNA-seq) analysis on STR-V-53-treated Hep-G2 cells, incubated at IC50 and 2× IC50 for 24 h, relative to the untreated (DMSO) control. Here, PDCD1 is linked to hepatocellular carcinoma.