As demonstrated in Figure 11, mice immunized with TgRPS2 protein, TgRPS2-PLGA, and TgRPS2-CS nanospheres exhibit reduced spleen infection by T. gondii. These findings suggested that nanospheres-immunization could provide immune protection in mice against toxoplasmosis, and such resistance could inhibit the growth of T. gondii tachyzoites to some extent. This evidence concerns the gene CS and toxoplasmosis.