IGFBP3 and Alzheimer disease: We also calculated differentially expressed genes (DEGs) from scRNA-seq data to compare basal_1 subtype in HDM-WT group against that in HDM-Par2-/- group and revealed the higher expression of Cd74, TSLP and IGFBP3 in HDM-WT group, indicating the participation of basal keratinocytes in the inflammation and immunomodulation of AD (Figure 3C).