To validate our DLK1 KO results, we assessed expression of NOTCH1, NE and adrenocortical differentiation proteins in an ACC PDO with high DLK1 expression (NCI-ACC48) and an ACC PDO with no DLK1 expression (NCI-ACC49) (Figure 4G). The gene discussed is DLK1; the disease is adrenal cortex carcinoma.