In contrast, HGB levels showed significant negative correlations with TMB in pan-cancer and in four individual cancer types in TCGA, including BLCA, BRCA, LUAD and PCPG (P < 0.05) (Figure 4B), and the significant negative correlation between HGB levels and TMB was also observed in the Pancancer_2020 pan-cancer (Supplementary Figure S1B). The gene discussed is CYGB; the disease is cancer.