miR-543, miR-301a-3p and miR-200b have been previously associated with CAD presentations including AMI.36–38 Additionally, miR-331 was reported to be a significant STEMI biomarker detected before myocardial necrosis markers (i.e., cTnI, miR-208 and -499)39 and miR-142-3p elevation was suggested as a potential biomarker for detection and diagnosis of STEMI.40 The elevated miR-543 and 301a in STEMI samples dropped after PCI, miR-543 levels even reached the control NCAD levels (Fig. 4d). Here, TNNI3 is linked to ST Elevation Myocardial Infarction.