Furthermore, certain crucial proteins related to proliferation (PCNA, cyclin-D1) and EMT (E-CAD, N-CAD, vimentin) were assayed, and the results indicated that the knockdown of TMEM209 inhibited the proliferation and metastasis of HCC cells by abrogating the expression of proliferation-associated proteins and EMT progression (Fig. 2G). Here, CCND1 is linked to hepatocellular carcinoma.