To validate above findings and to determine if APC possibly regulates eIF3a expression in vivo, we took advantage of the APCmin/+ mice, which have APC mutation and develop multiple adenomas [41], and tested eIF3a expression in the intestines of these animals in comparison with wild-type APC+/+ mice using quantitative RT-PCR and Western blot analyses. The gene discussed is APC; the disease is adenoma.