In this study, we not only showed that eIF3a expression is up-regulated in human colon cancer tissues and contributes to colon tumorigenesis, we also demonstrated that its expression is regulated by the Wnt/β-catenin signaling pathway and that inhibiting eIF3a expression can intercept APC mutation-induced tumorigenesis in intestinal tract. This evidence concerns the gene EIF3A and malignant colon neoplasm.