There were no significant changes in cytokines involved in type 1 immune responses (Figure S2D), chemokines (Figure S2E), serum antibodies (Figure S2F), or the immunoregulatory cytokines, TGF-β1(Figure S2G) and IL-10 (Figure S2H), during the controlled hookworm infection. This evidence concerns the gene TGFB1 and ancylostomiasis.