To further investigate the regulatory impact of the E7 virus on HDAC6 and DNMT3B in HPV-negative cervical cancer cells, C33A cells were separately infected with recombinant viruses expressing the E7 gene, including Ad4-HPV16E7, Ad4-HPV16E6E7, Ad4-HPV18E7, and Ad4-HPV18E6E7, while the Ad4-infected and non-infected C33A cells served as the control group. Here, DNMT3B is linked to cervical cancer.