tested samples of MPLC with lymph node metastasis (LNM) and found that the genes with the highest mutation frequencies in this pair were MUC16, FLNA, MUC4, FAT3, SETD2, and CALR, which implied that MPLC with LNM may have a unique mutation spectrum (47). This evidence concerns the gene MUC4 and metastatic malignant neoplasm in the lymph nodes.