ERBB2 and gastric cancer: With the utilization of WB and FACS and also by qRT-PCR and GEP analyses for evaluating the FASN protein and transcript expression level in both sensitive-resistant trastuzumab HER2+ GC cell lines under 2D and 3D culture conditions and found the FASN expression level was higher in 3D cultures, which was in collaboration with high CSC characteristics and worse prognosis.