DNA hypermethylation at the promoters of five tumor suppressor genes (RAAF1A, p16, DAPK, MGMT, and Rb) was examined in melanoma tumors, and 70% of the tumors had heterogeneous methylation patterns with methylation patterns of samples from the tumor core being more representative of the entire tumor than samples at the tumor edge [34]. This evidence concerns the gene RB1 and neoplasm.