Stimulations, including AFTV treatment, induce the recruitment of many T cell type TILs, consisting mainly of CD8+ T cells. High CD8 and PD-1 scores after the secondary surgery were significantly poor prognostic factors of survival after second resection as high PD-1 score (p < 0.05 each), while high CD3 score trended as a poor prognostic factor (p = 0.065). In tumor markers, high PD-L1 grading trended as a favorable prognostic factor of PFS second resection (p = 0.095). This evidence concerns the gene CD8A and neoplasm.