As hypothesised, western blot assays revealed the presence of both biomarkers in lysates of sEVs from serum purified by ultracentrifugation (Figure 7A, western blot analysis of ITGA4, ITGB2, and GADPH; B, normalised signal density by GAPDH), or TEI (Figure 7B, western blot analysis of ITGA4, ITGB2, and GADPH; Figure 7C, signal density of ITGA4 and ITGB2 normalised by GAPDH) with bands of higher intensity for T2high asthma compared to T2low asthma. Here, GAPDH is linked to asthma.