As hypothesised, western blot assays revealed the presence of both biomarkers in lysates of sEVs from serum purified by ultracentrifugation (Figure 7A, western blot analysis of ITGA4, ITGB2, and GADPH; B, normalised signal density by GAPDH), or TEI (Figure 7B, western blot analysis of ITGA4, ITGB2, and GADPH; Figure 7C, signal density of ITGA4 and ITGB2 normalised by GAPDH) with bands of higher intensity for T2high asthma compared to T2low asthma. This evidence concerns the gene ITGB2 and asthma.