Moreover, and as already reported in previous studies characterizing EV proteins in HCC, we confirmed that GGT1 [17] and MCFD2 [18] were present only in PHLF EVs in this study, and we speculate that in addition to the macroscopic grading of HCC, patient-specific HCC-molecular signatures may be present in EVs. The gene discussed is MCFD2; the disease is hepatocellular carcinoma.