Proteins with (paracrine) signal functions present in PHLF EVs (19) included leukocyte (ALCAM/CD166), T-cell, endothelial, and platelet activation cell surface glycoprotein CD109, the regulator of extracellular glutathione, GGT1 [17], or MCFD2, which is essential in the transport of the coagulation factors FV and FVIII [18] and described to be secreted in EVs in HCC [19], for example. The gene discussed is F8; the disease is hepatocellular carcinoma.