Next, we partially verified these candidates according to either KEGG analysis or gene-dependency analysis with Cancer Cell Line Encyclopedia (CCLE) BRCA cell lines, which leads to our identification of multiple genes, including PIP5K1A, NCKAP1, CYFIP1, DIS3, TBP, and EXOC1, as potent targets for enhancing MHC-I expression in breast cancer MCF7 cells. This evidence concerns the gene NCKAP1 and breast cancer.