Given that the C10orf90 gene is expressed at low levels in COAD, we utilized lentiviral constructs of two murine-derived colon cancer cell lines (CT26 and MC38) overexpressing the D7Ertd443e (C10orf90 homolog) gene to investigate the potential role of the D7Ertd443e gene in colon cancer. This evidence concerns the gene C10orf90 and malignant colon neoplasm.