This higher killing effect was associated with an increased replication, as confirmed by an increase of 30% in the number of copies detected in the OAdmCherry-treated HCC1937/luc2 supernatant (Figure 2C) and by western blot (WB) analysis with a higher expression of the adenovirus replication marker E1A, detecting a higher density of the protein in the HCC1937/luc2 cell line 24 h post-infection; this was especially noticeable at the lower molecular weight subunit of the protein (Figure 2D). The gene discussed is DHTKD1; the disease is infection.