CARD11 and colorectal carcinoma: In spite of these limitations, the following two points address such limitations to a large extent: (a) the analysis involved CARD11– and CARD11+ CRC patient samples, which is a better in vivo model with more intact TIME than the homogenous CRC cell line model, 3D organoid model, or the in vivo mouse model; and (b) two cell lines from different stages of CRC were used in this study to investigate the effect of CARD11 overexpression on CRC.