The top genes based on the gene frequency and significantly activated cellular pathways between CARD11- and empty vector-transfected CRC cell lines showed key genes, including EP300, STAT4, RB1, and HDAC2 for HCT-116 and CXCL1, CXCL3, CCL22, and IL1RN for HT-29. This evidence concerns the gene HDAC2 and colorectal carcinoma.