To investigate the role of ALDH1B1 in the clinical setting, a Spearman’s correlation coefficient analysis was performed in 507 lung adenocarcinoma samples which further validated that ALDH1B1 is statistically significantly positively correlated with many molecules involved in CSC-related (Wnt, Notch, Hedgehog, JAK/STAT, PIEK-PTEN/Akt) and metabolism-associated (glycolysis) signaling pathways, suggesting its crucial role in the CSC phenotype of lung adenocarcinoma cells. This evidence concerns the gene AKT1 and lung adenocarcinoma.