To determine whether the endonuclease activity contributes to the apoptosis-promoting effect of MCPIP1 in cervical cancer cells, we mutated the pLVX-TetOne-MCPIP1 plasmid by changing the aspartic acid (D) at 141 of the PIN domain into asparagine (N), generating pLVX-TetOne-MCPIP1-D141N (D141N), which expresses mutant MCPIP1 that almost lost the endonuclease activity [7]. The gene discussed is ZC3H12A; the disease is cervical cancer.