Conversely, at T0, both CCA and HCC patients show a tendency to have an increased percentage of CD8+ Treg cells (measured within CD8+ T cells), corresponding to 0.25 ± 0.22 and 0.37 ± 0.48, respectively (vs. 0.16 ± 0.21 in HG); and augmented CD8+ Tregs counts (CCA: 0.69 ± 0.83, HCC: 1.18 ± 1.17, HG: 0.59 ± 0.25), not reaching statistical significance (Supplementary Table S1). The gene discussed is CD8A; the disease is cholangiocarcinoma.