Regarding the functional characterization of CD4+ Tregs, no significant differences were observed among the groups under study for IL-10, TGFβ, and FoxP3 mRNA expression (Figure 1C), indicating that CD4+ Tregs from CCA and HCC have no functional differences from HG CD4+ Treg cells. This evidence concerns the gene TGFB1 and hepatocellular carcinoma.