ADAM17 and cardiomyopathy: To further testify the causal relation between ADAM17 and doxorubicin-induced cardiomyopathy, wild-type C57BL/6J male mice were transfected with adeno-associated virus (AAV) 9 carrying ADAM17 (AAV9-oeA17) to overexpress ADAM17 and adeno-associated virus vehicle as negative control (AAV9-oeNC) by means of tail vein injection in the third proportion of in vivo experiments (Fig. 3a).