ADAM17 deletion reduced the apoptotic response of cardiomyocytes in diabetic cardiomyopathy by activating AMPK pathways and improving autophagy process.14 In the present study, we examined the expression of autophagy-related proteins of p62 and LC3 in A17fl/fl + NS, A17fl/fl + DOX, A17α-MHCKO + NS and A17α-MHCKO + DOX groups and found no difference in p62 and LC3 expression among the four groups of mice (Supplementary Fig. 20a–c). This evidence concerns the gene ADAM17 and diabetic cardiomyopathy.