Several plasma-derived EV protein markers of melanoma have been previously suggested, for both the purposes of melanoma detection and monitoring of progression due to their alteration with advancing disease stages, including HSP70, CAV1, MCAM, MART-1, TYRP1, TYPR2, ITGA4, CSPG4, MIA, S100B, MET, TNC and PD-L1 [23,24,[27], [28], [29], [30], [31], [32]]. This evidence concerns the gene S100B and melanoma.