An ROC-AUC analysis of the four proteins showed AUCs ranging from 0.6 – 0.7 for each individual protein (Fig. 5E), and VWF and SERPIND1 were significantly increased in the samples obtained from patients with metastatic disease (VWF: W = 321.00, p = 0.007, SERPIND1: W = 343.00, p = 0.017, Mann-Whitney U test; Fig. 3.8F). There was no significant difference in the raw intensities of TNC (W = 377.00, p = 0.053, Mann-Whitney U test) or PLG (W = 419.00, p = 0.165, Mann-Whitney U test). Here, PLG is linked to metastatic neoplasm.