SASS6 and infection: To examine which developmental stage was impaired by 5-HT, we quantified male gametogenesis associated genes (the ribosomal gene of Plasmodium (18S), the sexual stage-specific actin isoform (Actin2), calcium-dependent protein kinase 1 (CDPK1), gamete egress and sporozoite traversal protein (GEST), protein phosphatase 1 (PPM1), basal body protein (SAS6), male development gene-1 (MDV1)) via qPCR 15 min post-infection and counted retort and ookinete numbers microscopically 12 h and 24 h post-infection respectively [18].