Previous studies demonstrated that cellular O‐GlcNAcylation affected the nuclear translocation of NF‐κB p65 in the progression of cholangiocarcinoma, leading to the activation of transcriptional activity.[12] Then we tested whether NF‐κB p65 is modified by O‐GlcNAcylation in the absence of LRP1 using succinylated wheat germ agglutinin (sWGA) pull‐down assay to specifically identify O‐GlcNAcylated proteins. Here, NFKB1 is linked to cholangiocarcinoma.