Figure 2D illustrates the relative RNA expression levels of these markers across all identified clusters. High expression levels mark the resident AM clusters as Itgax+Adgre1+ were predominantly observed in the sham condition. Following PA infection, a new cluster expressing Itgam+Ly6c2+Ly6g+ begins to emerge, representing newly recruited myeloid-derived cells, despite the relatively moderate expression of Ly6c2 and Ly6g genes. This evidence concerns the gene ITGAM and infection.