GCG and type 1 diabetes mellitus: The median cytoplasmic area was reduced in glucagon-positive cells (96.07 μm2 vs 75.15 μm2 vs 76.37 μm2 for non-diabetic donors vs donors with short-duration type 1 diabetes vs donors with long-duration type 1 diabetes, respectively) (Fig. 2f) while the nuclear area remained comparable in all donor groups (Fig. 2g).