Western blot analysis was used to measure the expression levels of the mitophagy indicator microtubule‐associated protein 1 light chain 3 (LC3), selective autophagy receptor SQSTM1/p62, the mitochondrial outer membrane indicator TOMM20 and GLS2 in the control, AAV‐con+KA, and AAV‐GLS2 + KA groups to examine the impact of GLS2 on mitophagy during the development of epilepsy. The gene discussed is TOMM20; the disease is epilepsy.