Due to a lack of reliable and high‐grade antibodies against TBX2 and TBX3 for immunofluorescence, this study used a tetracycline‐on (Tet‐On) system that enabled the inducible expression of 3XFLAG‐tagged TBX2 or 3XFLAG‐tagged TBX3 in the 501mel human melanoma cell line. The gene discussed is TBX3; the disease is melanoma.