We classified the 40,650 astrocytomas obtained by Seurat and named the seven subclusters according to the marker genes as C0 IGFBP7+ Glioma cells, C1 OLIG2+ Glioma cells, C2 LINC02283+ Glioma cells, C3 LINC00632+ Glioma cells, C4 MX1+ Glioma cells, C5 FOSB+ Glioma cells, and C6 DLL3+ Glioma cells. The gene discussed is OLIG2; the disease is glioma.