IGFBP7 and astrocytoma (excluding glioblastoma): Figure 2A violin plots demonstrated the expression levels of the named genes of the subclusters in each subcluster, and interestingly, IGFBP7, the named gene of the C0 subcluster, was also expressed in the C4 subcluster. We visualized the DEGs (differential expressed genes) in each subcluster of astrocytoma using volcano plots (Figure 2B).